Nano Zinc Oxide Inhibits Fibrillar Growth and Suppresses Cellular Toxicity of Lysozyme Amyloid

Deposition of amyloid fibers has been a common pathological event in many neurodegenerations, such as Alzheimer’s disease, Parkinson’s disease, and Prion disease. Although various therapeutic interventions have been reported, nanoparticles have recently been considered as possible inhibitors of amyloid fibrillation. Here, we reported the effect of three different forms of zinc oxide nanoparticles (ZnONP): uncapped (ZnONPuncap), starch-capped (ZnONPST), and self-assembled (ZnONPassmb) (average sizes of 10, 30, and 163 nm, respectively), having a core size of 10–15 nm, in the amyloid growth of hen egg white lysozyme (HEWL). We monitored the amyloid growth by electron microscopy as well as Thioflavin-T (ThT) measurement. We observed that ZnONP demonstrated a dose-dependent inhibition of fibrillar amyloid growth of HEWL, with the greatest effect being exhibited by ZnONPST. Such inhibition was also associated with a decrease in cross β-sheet amount, surface hydrophobicity as well as increase of stability of pr...