Determination of Sulfadimethoxine Residues in Skunk Serum by HPLC

Sulfadimethoxine (SDM) was extracted from skunk serum and isolated by reversed-phase high performance liquid chromatography. SDM was detected by ultra-violet absorbance at 270 nm and quantified by comparison to an external cali- bration standard. Recovery data were determined by analyzing SDM fortified control serum. The overall mean recovery with relative standard deviations of SDM in fortified skunk serum samples was 99 + 7%. The recovery for 0.38, 5.2, and 14.2 mg/mL SDM was 96.0+ 7.5%, 102 + 6.1%, and 97.3+ 5.1%, respectively. The method limit of detection for SDM in skunk serum ranged from 0.032 to 0.057 mg/mL SDM with a mean value of 0.040 mg/mL SDM. The method reported is much simpler and equally efficient as previous methods developed for the determi- nation of SDM residues in serum.