[Specific anti-tumor effect of immune competent cell induced by HSP-70 of HeLa cells in vitro].

Background Objective: Recent studies indicated that heat shock proteins (HSPs) are associated with antigenic peptides and participate in the antitumor T cell response in animals.This study was designed to investigate the mechanism of anti tumor effect of immune competent cells (ICC) induced by HSP 70 of HeLa ( cervical carcinoma ) cells in vitro. Methods:The normal peripheral blood mononuclear cells(PBMCs) stimulated with HSP 70 of HeLa cells in vitro.T cell phenotypes were analyzed before and after stimulated by PBMCs. HSP 70 primed the immune competent cells (ICCs) from PBMCs were tested for cytotoxic activity against HeLa cell. Results: CD3+ cell was 85.73±1.44%,up from 57.68±1.46% before the amplification(P 0.002).CD8+ cell was 48.06±1.66%,up from 23.56±1.86% before the amplification(P 0.002). The ICCs induced by HSP 70 were shown significant cytotoxic activity (82.69±1.97%,62.11±1.61%)against HeLa cells and cervical carcinoma cells,and the cytotoxic activity (31.05±2.09%)of the ICC against HeLa cells could be blocked by anti HSP 70 antibody. Conclusion: The ICC can be induced selectively by PBMCs with HSP 70 of HeLa cells, and has specific cytotoxic activity against HeLa cell.