Expression and clinical significance of genetic amplication of the human telomerase component gene in cervical adenocarcinomas

Objective To detect the expression and clinical significance of amplification of the human telomerase component(hTERC) in the specimens of cervical adenocarcinomas and discuss the clinical significance of its normal amplification. Methods Retrospective analysis was carried out to the clinical material of 15 cervical adenocarcinomaspatients who received operation in our hospital from 2000 to 2010. Their paraffin-embedded cervical tissues were established as experimental group,15 cervical specimens of chronic cervicitis women were established as control group. All of specimens were detected by FISH to evaluate the hTERC gene amplification. Results No control group samples revealed copy number increases of 3q, while 100 ％ of cervical adenocarcinomas tissues showed extra copies of 3q. hTERC copy numbers in cervical adenocarcinomas were significantly higber than those in control group 1 samples, (P＜0.001).Conclusions hTERC gene showed abnormal amplification in the cervical adenocarcinomas. And the detection of hTERC gene amplification by FISH could help the early diagnosis of cervical adenocarcinomas. Key words: Cervical adenocarcinomas;  hTERC gene;  Fluorescence in situ hybridization; High risk of human papillomavirus infection