And a method of detecting biologically active composition

The present invention provides compositions comprising water, it can be converted by biological activity to a first indicator reagent composition and a first plurality of particles of a first biological derivative. The first indicator reagent can comprise a fluorogenic enzyme substrate having a fluorophore, the fluorophore is selected from umbelliferone, 7-amino-coumarin, naphthylamine [beta], [beta] naphthol, fluorescein, halogen test Ling, (1,3-dichloro-9,9-dimethyl-acridine-2-one), rhodamine 110 9H- composition, derivatives and any of the foregoing any of the foregoing fluorophores fluorophores. The particles are able to accept and retain the first biological derivative from the aqueous liquid. The first biological derivative may indicate a microorganism. The composition may also comprise a gelling agent. The present invention also provides the use of the composition in a sample to detect the presence or absence of microorganisms.