The effect of Dilazep on F10 cells in vitro.

Experiments on F10 cell growth, colony ability, cell adhesion and ultramorphology at SEM have been performed. The effect of Dilazep (DIL) has been compared with that of well known modulating agents such as Flunarizine (FLU) and Verapamil (VER) on cells cultured in high Ca ++ medium (HCM) and in low calcium medium (LCM). While in HCM there is no difference among the three drugs, FLU and VER had a stronger effect on cell growth inhibition in LCM. Cell adhesion to the growth substratum, evaluated by the technique of spontaneous detachment in culture medium, is reduced in DIL treated cells in comparison to the controls