Callus induction and viability of mangosteen (Garcinia mangostana L.) from in vitro bud

Mangosteen is a unique tropical fruit that originated from Indonesia that has a good prospect. One of the main problem is the slow rate of growth of this plant, so it is difficult to get seed. The need to produce numerous and good quality plantlet in a short time has been carried out with in vitro culture. To solve the problem is getting from callus induction by tissue culture method. The objective of this research was to find the best combination of medium and plant growth regulator for in vitro mangosteen callus induction. In vitro bud were treated with six level treatment, a) without plant growth regulator, b) 1 ppm 2,4 D, c) 1 ppm 2,4 D and 1 ppm Kinetin, d) 1 ppm 2,4 D and 0.5 ppm TDZ, e) 1 ppm Kinetin and 0.5 ppm TDZ, f) 1 ppm 2,4 D and 1 ppm Kinetin and 0.5 ppm TDZ for callus induction in Murashige and Skoog (MS) medium. After five week treatment, callus is transfered to 0.5 ppm NAA + 0.2 ppm Kinetin + five concentrations of Benzyl Amino Purine (BAP) (0, 1, 2, 3, 4 ppm). Our result show that plant growth regulator positively affected mangosteen callus induction (time of callus appearance, biomassa, colour, texture, browning). The best medium for mangosteen callus induction was found to be MS + 1 ppm Kinetin + 0.5 ppm Thidiazuron. After transfer, colour, size and texture of the callus is changes, but not for biomassa.