Establishment and optimization of RAPD technique in Pyrus L.

Tested with Yali pear and Betulaefolia,the paper studied the extract of DNA of Pyrus and the optimized RAPD reaction system.The result showed as follows:by raising the concentrations of antioxidative components and antiphenolic compounds(2% β-ME,2% PVP-40,20 mmol/L Na2S2O5),the purity of the extracted genomic DNA was higher.It's concluded that the optimum reaction system that contained Mg2+ 2.0 mmol/L,dNTPs 200 μmol/L,primer 0.4 μmol/L,1.6 ng/μL template DNA,0.06 U/μL,Taq DNA polymerase would be applied in the amplification of RAPD.It explained that reformed CTAB method could extract the genomic DNA of Pyrus successfully,and the optimum RAPD reaction system could amplificate RAPD in Pyrus L..