In vitro infection of human umbilical cord blood CD34+ hematopoietic progenitor cells by HIV-1 CRF07_BC enveloped pseudovirus.
2012
To determine whether CRF07_BC, one of the most predominant strains that accounts for one third HIV-1
prevalence in China, has the ability to infect hematopoietic progenitor cells (HPCs), human Umbilical Cord Blood (UCB)
derived CD34+ HPCs isolated with high purity were infected by HIV-1 pseudotyped with CRF07_BC envelope. After
HIV-1 infection, ~0.86% CD34+ HPCs were co-stained for CD34 and intracellular HIV Gag. HIV p24 antigen was
detectable and reached maximal release between day 2-4 after HIV-1 infection. The data of nested Alu-LTR PCR proved
the integration of HIV-1 genome into the host genome occurred in HIV-1-infected HPCs. These data demonstrated that
the envelope of CRF07_BC from China has the capability of resulting in infection to CD34+ HPCs, which may serve as a
mechanism for long-term latency of HIV-1 infection in vivo.
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