Antimicrobial efficacy of a very stable hypochlorous acid formula compared to other antiseptics used in treating wounds. In vitro study on microorganisms with or without biofilm

Abstract Background Many antiseptics have been used to treat wounds. Aim To compare the microbicidal efficacy of ClHO (Clortech R) with other other antiseptics used on wounds, healthy skin and mucous membranes. Methods We study the microbicidal efficacy of 13 antiseptic products on 8 microorganisms (3 Gram-positive;3 Gram-negative; 2 yeasts) inoculated on organic germ-carriers. In addition, the loss of efficacy against Staphylococcus aureus and Pseudomonas aeruginosa with biofilm was assessed with the 6 best-performing products. Findings 1% Chlorhexidine had the highest microbicidal effect at 1 minute. At 5 minutes, 500 and 1500 mg/L ClHO showed similar, or better, activity than the other antiseptics studied. The ClHO concentration of 300 mg/L achieved this same efficacy at 10 minutes. The product that lost the most efficacy due to biofilm was 1% Chlorhexidine, while 1% PVP-I and ClHO at either 300 or 500 mg/L were moderately affected by biofilm. The most effective in the presence of biofilm was ClHO at 1500 mg/L. Conclusions ClHO at medium-low concentrations (300 or 500 mg/L) is a good antiseptic that can be used on wounds and mucous membranes for 5-10 minutes. Lower concentrations of ClHO, as well as of the other antiseptics studied, were less effective or more altered by the biofilm. ClHO at a concentration of 1500 mg/L is very effective in presence or absence of biofilm that can be used on healthy skin for 5 minutes.