Mapping a leaf senescence gene els1 by BSR-seq in common wheat
2018
Abstract Leaf senescence is normally the last stage of plant development. Early senescence of functional leaves significantly reduces the photosynthetic time and efficiency, seriously affecting grain yield and quality in wheat. Discovering genes responsible for early leaf senescence ( els ) are necessary for developing novel germplasms and cultivars with delayed leaf-senescence through molecular manipulation and marker assisted selection. In this study, we identified an early leaf senescence line M114 in a derivative of a wheat breeding population. Genetic analysis indicated that early leaf senescence in M114 is controlled by a single recessive gene, provisionally designated els1 . By applying bulked segregant analysis and RNA-Seq (BSR-Seq), seven polymorphic markers linked to els1 were developed and the gene was located on chromosome arm 2BS in a 1.5 cM genetic interval between markers WGGB303 and WGGB305 . A co-segregating marker, WGGB302 , provide a starting point for fine mapping and map-based cloning of els1 .
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