Physiological Studies on Development Flower Bud In vitro of 'Le Conte' Pear Trees

2 Abstract: Flower bud explants from Le Conte pear were cultured individually on MS medium supplemented with 0.1 mg/L 6-benzylaminopurine (BAP), 30 g/L sucrose and 7.0 g/L Difico Bacto agar. Different root pruning treatments (0.0, 30, 60 and 90 cm) from the trunk of mother plants during bud break and full bloom stages and Medium strength (Full, one- half and one-fourth) were tested. Data indicated that bud break stage surpassed full bloom stage in all parameters under study (shoot length, number of node, number of branch, explants development, fresh weight, number of leaves and greening). Moreover, using root pruning treatments (30 cm) was better than (0.0, 60 and 90 cm). Also, one- half treatment enhanced the proliferation percentage and shoot number compared with the other treatments.