Nanometer PCR kit for detecting Newcastle disease virus and application of nanometer PCR kit

2015 
The invention discloses a nanometer PCR kit for detecting Newcastle disease virus and application of the nanometer PCR kit. The kit comprises a 5x inverse transcription buffer solution, a dNTP Mixture, inverse transcriptase, an RNA enzyme inhibitor and inverse transcription primers; the kit is characterized by also comprising a 2xNanoPCR Mix, a forward primer and a reverse primer, wherein the sequence of the forward primer is shown in DEQ ID No.1; the sequence of the reverse primer is shown in SEQ ID No.2. The kit can be used for detecting the Newcastle disease virus. The invention also provides a method for detecting the Newcastle disease virus by adopting the kit, the kit can be used for rapidly and specifically detecting the Newcastle disease virus, so the detection efficiency of the Newcastle disease virus is greatly improved and the specific amplification yield of the Newcastle disease virus is greatly increased.
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