[Pyrophosphate-dependent inactivation of tyrosyl-tRNA synthetase during aminoacylation of heterologous tRNA].
1992
: It has been shown that heterologous aminoacylation of tRNA by tyrosyl-tRNA synthetase leads to inactivation of the enzyme. Inorganic pyrophosphatase prevents the inactivation and increases the enzyme activity and aminoacylation level in a heterologous system. A putative inactivation mechanism is discussed.
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