HindIII polymorphism in the human c-sis proto-oncogene
1988
The 2.0 kb BamH1 restriction fragment corresponding to a cDNA insert encoding the human c-sis PDGF A chain and nucleotide sequences homologous to the v-sis gene was isolated from plasmid pSM-1. An identical polymorphism was noted using the 1.2 kb PstI fragment or the 1.0 kb PstI/XbaI fragment isolated from the v-sis sequence subcloned in the plasmid pV-sis. HindIII identifies a single bi-allelic polymorphism with bands at 22.6 kb and 19.4 kb. Co-dominant segregation was demonstrated in 1 family. This polymorphism is not easily detected unless the restricted DNA is separated on 0.6-0.8% agarose gels. Resolution was optimal if gels were run until the bromophenol blue tracking dye had migrated 14 cm from the origin and Southern blotting was performed under alkaline conditions.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
0
References
11
Citations
NaN
KQI