Disposable Polydimethylsioxane Package for 'Bio~Microfluidic System'

A disposable polydimethylsioxane package is developed for ‘Bio-Microfluidic System’. Disposable Bio-Microfluidic system avoids the contamination of the system after each use and this requires the use of low cost materials. Polydimethylsioxane (PDMS) is an attractive low cost material for making substrates of the micro-fluidic package. The disposable PDMS Package consists of PDMS substrates, which are fabricated by soft-lithography. The PDMS substrates are bonded together to form the disposable package with the use of a thin-film, coated both side with adhesive material. The adhesive material is selected by chemical soaking test, peel test and bio-analysis. The tests reveal that the adhesive material can handle all chemical reagents without causing blockage or discoloration to the package. The disposable PDMS package uses a plug-in concept as the macro-micro fluidic interconnects that allows easy detachment of package from the external fluidic system. The developed disposable PDMS package has demonstrated a fluidic leak-proof package that handles up to 100kPa pressure with flow-rate of 200µl/min. The package has also demonstrated that it can filter out Viral Ribonucleic Acids (RNA) from spiked blood sample. This micro fluidic package forms an integral part of the bio micro fluidic system.