Rapid, automated analysis of monosaccharides by high-performance anion-exchange chromatography of borate complexes with fluorimetric detection using 2-cyanoacetamide.

Abstract Biochemically important monosaccharides were rapidly separated by automated chromatography of their borate complexes on columns packed with newly developed anion-exchange resins, and sensitively detected by fluorimetric postlabeling with 2-cyanoacetamide. Optimization studies for stepwise, gradient, and single-buffer elutions are described, and the reproducibility of the determination of such monosaccharides is discussed. A few examples of the application of this rapid, sensitive automated analysis are also presented.