P-A11 An End-To-End Solution in Deep Sequencing of HIV: Sensitive Detection of HIV-1 Genomic Variations Combined with Advanced Genotyping Downstream Analysis System

To detect HIV minority populations of resistant virus, a hyper sensitive test is needed, and today’s Gold Standard, Sanger sequencing, is not able to detect minority population less than 10 to 20 percent of the virus population. Ultra-deep sequencing on the Miseq instrument allows the identification of HIV nucleotide variants and variant haplotype signatures present at <1% in samples. In this study, samples were sequenced on the Miseq and uploaded onto BaseSpace to be analyzed through the DeepChek HIV Pipeline. (Fig. 1).