Site-Specifically Albumin Conjugated Urate Oxidase and The Method for site-specifically conjugating albumin to Protein

The present invention relates to a method of connecting the location-specific albumin as a position-specific urate oxidase protein and albumin are linked. In the present invention, it was confirmed that the substitution of a tryptophan residue uric acid oxidase with unnatural amino acids, and if the connecting the location-specific albumin as herein, in vivo activity of the urate oxidase is maintained while the half-life is increased. Therefore, when possible to improve the half-life of the proteins difficult to treat effectively connected to albumin using the method for connecting the albumin to a therapeutic protein of the invention.