A Study on the Enzymatic Activity in Flue-cured Tobacco Leaf in Different Areas during the Aging Stage

In order to explore the changing laws of the activity of the polypgenol oxidase,peroxidase,lipoxygenase and phenylalanine ammonialyase in the flue-cured tobacco leaves during the aging stage in different areas under the Henan atmosphere condition.The results showed that:(1) at different aging times,the activity of every enzyme gradually increased in the beginning period,and the amount peaked at the aging of the 6th or 9th months,then diminished gradually;(2) the activities of enzymes in different areas had different decreading amplitudes during the 24 months course of aging,the decreading amplitudes of the activity of POD and PAL were the largest in Hunan area,they decreased by 77.01% and 4.15% respectively;the activity of PPO in Yunnan area had the largest decreading amplitude,and reached 66.67% ;the activity of LOX in the flue-crued tobacco leaves in Guizhou had the largest decreading amplitude(68.93%);(3)the activity of every enzyme in the flue-cured tobacco leaves in Zimbabwe decreased gradually during the 15th stage from the to the 39th months and the activity was comparatively lower,especilly the activity of LOX had the largest decreading amplitude.