Determination of icariin in Shenglixiong Capsule by RP-HPLC

Objective To establish a RP-HPLC method for the determination of icariin in Shenglixiong Capsule.Methods The Inertsil ODS-SP column(4.6 mm×250 mm,5 μm) was adopted with acetonitrile-water-phosphoric acid(28∶ 72∶ 1) as mobile phase,the flow rate was 1.0 mL·min-1 and the detection wavelength was 270 nm.Results The icariin showed a good linear relationship within the range of 0.172 0~1.720 μg(r=0.999 9),the average recovery was 97.0% and the RSD was 1.2%(n=6).Conclusion The method was simple,rapid and accurate with good reproducibility,and it can be used for the quality control of Shenglixiong Capsule.