Preliminary study on coupling between extraction and acetone-butanol fermentation to increase butanol production

By knowing the biocompatibility of the five kinds of extractant system,such as oleyl-20% decanol alcohol,oleyl-40% decanol alcohol,1-octanol,1-heptanol and ethyl acetate,a mixed extractant that contains decanol in oleyl alcohol is chosen for its low toxicity to the strains.After measuring the extraction rate of oleyl-decanol alcohol,phase rate of 1∶5 and a mixed extractant that contained 20% decanol in oleyl alcohol are combined to carry out in-situ extractive acetone-butanol fermentation,resulting 19.21 g/L of butanol concentration.Butanol productivity could be 62.8% higher than that of control,meanwhile,comparing with the control,total organic solvent productivity increases by 42.3%.