Genome-wide approaches to monitor pre-mRNA splicing.
2010
Abstract Pre-mRNA processing is an essential control-point in the gene expression pathway of eukaryotic organisms. The budding yeast Saccharomyces cerevisiae offers a powerful opportunity to examine the regulation of this pathway. In this chapter, we will describe methods that have been developed in our lab and others to examine pre-mRNA splicing from a genome-wide perspective in yeast. Our goal is to provide all of the necessary information—from microarray design to experimental setup to data analysis—to facilitate the widespread use of this technology.
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