Functionality study of Na6PMo11FeO40 as a mushroom tyrosinase inhibitor

Abstract The inhibitory effects of iron-substituted phosphomolybdic acid (Na 6 PMo 11 FeO 40 , abbreviated as PMo 11 Fe) on mushroom tyrosinase were investigated. The Native-PAGE results show that PMo 11 Fe has an inhibitory effect on tyrosinase. A spectrophotometric analysis shows that PMo 11 Fe is a reversible and noncompetitive inhibitor with K I  =  K IS  = 0.47 mmol L −1 . The effects of PMo 11 Fe on the preservation of lotus root slices were studied. The results show that PMo 11 Fe can significantly slow the browning rate of lotus root slices, inhibit the activity of polyphenol oxidase (PPO), enhance the activity of peroxidase (POD) and superoxide dismutase (SOD), maintain high levels of glutathione (GSH), ascorbic acid (ASA) and sucrose and control the peroxidation degree of fresh-cut fruits and vegetables. This study may help to elucidate the design of mushroom tyrosinase inhibitors of polyoxometalates (abbreviated as POMs) and a theoretical basis for the use of POMs to inhibit fruit spoilage.