High-resolution three-photon biomedical imaging using doped ZnS nanocrystals

The application of three-photon excitation to biomedical imaging is demonstrated by combining the three-photon excitation properties of ZnS nanocrystals and the visible emission from Mn2+ dopants. The biocompatible, doped nanocrystals are used for high-resolution cellular imaging and in vivo tumour-targeting imaging under non-invasive conditions.