Method for measuring adhesion force between pollen and stigma by using centrifuge method

The invention discloses a method for measuring an adhesion force between pollen and stigma by using a centrifuge method. The method comprises the following steps of pollen density detection, centrifugate preparation, fixation of a pollinated stigma, differential centrifugation elution, fluorescence microscopic examination, adhesion force calculation and the like. The pollen density is measured by a density gradient centrifugation method at first; secondly, a cane sugar solution with a mass percent of 70% is prepared to serve as centrifugate; thirdly, the pollinated stigma to be detected is fixed at the bottom of a centrifuge tube; at a temperature of 40-30 DEG C, the pollinated stigma which is dyed with water-soluble Benzedrine blue with the mass percent of 0.01-1%, is eluted in a centrifugal machine for 5-20min; and centrifugal acceleration when the pollen on the pollinated stigma to be detected is eluted is observed under a microscope; and the adhesion force between the pollen and the stigma is calculated according to the following formula shown in the description. The method provided by the invention can be used for measuring the adhesion force between various pollens and the stigma, and has the advantages of high measurement precision, good repeatability, simplicity and convenience, and easiness in operation.