Study of the survivin expression in pancreatic carcinoma cell line PANC-1 induced by gemcitabine

Objective To study the relationship between chemoresistance and the expression of survivin,induced by gemcitabine,in pancreatic carcinoma cell line PANC-1.Methods The pancreatic carcinoma cell line PANC-1 was cultured by RPMI 1640 and gemcitabine with the concentration of 1 μg /ml and 10 μg /mL respectively,and survivin expression was examined by RT-PCR and Western blot.The relationship of the survivin level of the cell and its chemoresistance to gemcitabine was analyzed.Results Tweenty-four and 48 hours after culture with gemcitabine concentration of 1 μg /ml and 10 μg /mL respectively,survivinmRNA level was increased(1.34±0.12),(2.40±0.17),and(3.33±0.20),(4.41±0.18) folds,and the protein expression was enhanced(1.20±0.07),(1.48±0.19),and(2.90±0.04),(4.50±0.20) folds respectively.There was a significant difference between the two groups(P0.05).Conclusions The intensity of pancreatic carcinoma cell lines chemoresistance could be enhanced by the action of gemcitabine on the up-regulated expression of survivin.