Computer simulation of ESR signals of liver paramagnetic centers

Changes of paramagnetic centres concentration characterized by g-factors values of 1.94, 2.2, and 2.03 in the rat liver were studied by ESR method under acute intoxication by diethylnitrosamine (DENA) and at preliminary threefold treatment of animals with butylhydroxytoluene (BHT). A protective effect of BHT can be explained by its stabilizing action of the membrane structures. A comparison has been carried out with a similar study of paramagnetic centres in the experiment of chronic intoxication by DENA. A simulation was performed of the liver tissue ESR spectra by means of special computer program. The parameters of simulated ESR spectra of the liver tissue with due regard for ESR signal g 2.03 corresponded to the parameters of the experimental spectra. Confirmations were obtained for the nature and number of paramagnetic centres in the liver tissue.