Quantitative analysis on inhibiting effects of siRNA on VEGF-C expression in EC9706 cells in vitro and in vivo

Objective To explore the inhibition of vascular endothelial growth factor C(VEGF-C) expression by small interfering RNA(siRNA) in human esophageal cancer EC9706 cells in vitro and in vivo.Methods Three self-designed and constructed VEGF-C-targeted siRNA eukaryotic expression vectors were introduced into esophageal carcinoma EC9706 cells via liposome.Expressions of VEGF-C protein in EC9706 cells and in transplanted tumor cells in nude mice were observed by S-P immunohistochemistry and assessed quantitatively by HPIAS-1000 image analysis system before and after transfection,respectively.Results There were lots of positive granules in control EC9706 cells and in cells transfected with un-matching any known human coding mRNA.There was no significant difference between them(P0.05).The cells transfected with VEGF-C-targeted siRNA expressed few positive granules.There was a significant difference between the control EC9706 cells and in the cells transfectied with siRNA(P0.01).Conclusion The specific siRNA of VEGF-C can significantly inhibit the expression of VEGF-C in EC9706 cells in vitro and in vivo.The siRNA against VEGF-C may become a potential method of treatment for lymphatic metastasis in human esophageal cancer.