Friable Callus Induction of Fujian Wild Grapes(Vitis Amurensis Rupr.) and Its Subculture and Maintenance

The plantlets of Fujian wild grapes(Vitis amurensis Rupr.) were established by using its stems with node as explants.And its callus induction,subculture and long-term maintenance were carried out by using the stems and leaves of these plantlets.The results showed that,with the illumination of lights,the effect of medium MS+BA 2.0 mg·L-1+NAA 0.2 mg·L-1+ Sucrose 30 g·L-1 revealed the best on inducing of calluses for a rate of 100%.The medium MS+2,4-D 1.0 mg·L-1+ Sucrose 30 g·L-1 was the best for the callus subculture multiplication to obtain the friable calluses in good growth.For a long-term maintenance,the calluses were sub-cultured for several generations with the medium MS+2,4-D 1.0 mg·L-1+ Sucrose 30 g·L-1 and then followed with the sub-culture of medium MS+2,4-D 1.0 mg·L-1+ KT 0.5 mg·L-1+AgNO3 5.0 mg·L-1+ Sucrose 30 g·L-1 for one generation.Continually,the calluses were alternately sub-cultured with the two mediums and could grow vigorously with the illumination of lights.