Use of fluorescence to determine reduction in Bemisia tabaci (Hemiptera: Aleyrodidae) nymph feeding when exposed to cyantraniliprole and imidacloprid through systemic applications

Abstract The Bemisia tabaci whitefly is an important pest of many agricultural crops. Direct feeding by this pest can cause physiological plant symptoms including irregular ripening in tomatoes and silverleaf disorder in squash. In addition, B . tabaci can transmit more than 100 plant viruses that cause severe diseases that affect crop yield and quality. Insecticides are often applied to control this pest and they may be applied to the foliage and/or to the soil. Insecticides that kill quickly or cause feeding cessation reduce the damage caused by B . tabaci on the host plant most effectively. In this study fluorescence was used to assess B . tabaci feeding and to determine the effect of systemically applied insecticides on feeding by the pest. Cyantraniliprole (Verimark™) and imidacloprid (AdmirePro ® ) produced a rapid reduction in B . tabaci feeding. At 24 h after a systemic application, the percentage of whitefly nymphs still feeding in plants treated with cyantraniliprole (anthranilic diamide) and imidacloprid (neonicotinoid) were 19% and 33%, respectively. Both products showed a good reduction in insect feeding and since they have different modes of action they should be considered as rotational partners for B . tabaci control in areas where there is no resistance to neonicotinoid insecticides. While the fluorescein sodium salt cannot be used to trace the uptake of insecticides, it has been shown to reliably demonstrate feeding cessation of whitefly nymphs after foliar and systemic applications of insecticides.