Determination of efficacious components of Gentianaceae plants and their calli by RP-HPLC

A developed reverse phase high performance liquid chromatography(RPHPLC) was used to determine the contents of five efficacious components(Swertiamarin,Gentiopicroside,Mangiferin,Oleanolic acid and Urolic acid) in Chinese medical herbs Swertia musstii Franch,Gentiana siphonantha Maxim,and Gentianella albiflora Maxim.,Gentianopsis paludosa Ma.and their calli from subculturals.A Phenomenex Luna C18 column was used and the column temperature was room temperature.Swertiamarin,Gentiopicroside and Mangiferin were detected at 259 nm with Vmethanol∶Vwater∶Vacetic acid=25.00∶75.00∶0.04 as mobile phase and 0.8 mL/min as flow rate.While by Vmethanol∶Vwater∶Vacetic acid=85.00∶15.00∶0.04 as mobile phase,1.0 mL/min as flow rate and 209 nm as wavelength,and the contents of Oleanolic and Urolic acid were analyzed.The kind and content of efficacious components from the four Chinese medical herbs were quite different.Swertia musstii Franch contained the most efficacious components and its contents were the highest.The contents of five efficacious compounds in the above four medical herbs calli had less efficacious compounds than that of wild plants and their changes were different.However,according to the projected growth rate,the content of efficacious components accumulation of the callus was much higher than that of the wild plant.This is a precise and quick method to analyze the efficacious components in Chinese medical herbs,which provides the basic for large-scale production of efficacious components in Chinese medical herbs.