SPR study for analysis of a water-soluble glycopolymer interface and molecular recognition properties

A glycopolymer with different mannose incorporation ratios was synthesized via a reversible addition fragmentation chain transfer with acrylamide. The polymers were immobilized onto a gold substrate by the thiol terminate. The glycopolymer layer structures and the glycopolymer–protein interactions were analyzed using a surface plasmon resonance technique. The glycopolymers formed pancake-like structures under dry conditions and were swollen in water. The interaction between the glycopolymer and the protein was enhanced by the cluster effect of the mannose units.