Effect of Jagged-1 on the cytokine expression of murine myeloid-derived dendritic cells

AIM:To investigate the in vitro effect of soluble Jagged-1/Fc on the cytokine expression of murine myeloid-derived dendritic cells induced by both rmGM-CSF and rmIL-4.METHODS:The DC culture model under rmGM-CSF plus rmIL-4 conditions was established.The influences of Jagged-1/Fc on the morphology of DC was checked,and the cytokine expression were analyzed by LuminexTM system and ELISA.The activation capacity of Jagged-1/Fc-induced DC for allogeneic lymphocytes was detected by MTT assay.RESULTS:Compared with LPS or Zymosan A,the amount of IL-4 expression of Jagged-1/Fc-induced DC increased significantly and the amount of TNF-α expression decreased markedly but γ-secretase inhibitor DAPT reversed the inhibition of Jagged-1/Fc on TNF-α expression from DC and returned it to the control level.Jagged-1/Fc altered the amount of IL-10,IL-6,IL-2,IL-12 and IFN-γ expression unconspicuously and had no effect on TGF-β expression.The mixed lymphocyte reaction(MLR)revealed that Jagged-1/Fc-induced DC had the weakest ability to activate allogeneic lymphocytes and LPS-induced DC showed the strongest activation ability.CONCLUSION:The results indicate that Jagged-1/Fc-induced DC incline to induce immune tolerance and to prime nave T cells to the Th2 cells.