Detection of Clamydia trachomatis with ligase chain reaction.

Objective To investigate the age distribution of the active trachoma and evaluate the significance of trachoma classi-ficltion and ligase chain reaction. Methods 89 cases were investigated and classified by WHO trachoma classification, samples of the superior conjunctiva were collected for detection of Chlamydia trachomatis with LCR. Results Trachoma is more common in the age group below 10-year-old (56.18% ) .It is 26.94% in the age group below 20-year-old.Out of 84 trachoma and suspected trachoma,16 cases are LCR positive(19.05% ) .The positive rates in various sites are 7.14% - 34.38% . Conclution Attention be paid to the prevention and treatment of adolescent trachoma; WHO trachoma classification is easy and feasible in the countryside . Ldgase chain reaction could be used for early diagnosis and the detection of a large number of sampes. Attention be paid to the collection of samples and the temperature of transportation should be controlled carefully.