HL-60 세포의 유전자 발현 및 topoisomerase의 기능 활성에 미치는 억제제의 영향

This studies were designed to elucidate whether inhibitors of topoisomerase regulate function and activity of topoisomerase, and gene expression in HL-60 human leukemia cells. HL-60 cells were treated with 10-hydroxycamptothecin or doxorubicin, total RNA was isolated, and expressed genes were investigated with human oligonucleotide microarray containing 10K gene, respectively. Expression profiles of the human leukemia HL-60 cells treated with 10-hydroxycamptothecin (10-CPT) or doxorubicin associated with signal transduction, cell adhesion, cell cycle, cell growth, cell proliferation, cell differentiation, transcription and immune response, especially genes related with transcription and cell growth. In HL-60 cells treated with 10-CPT, the expression of topoisomerase Ⅲα, Ⅲβ and Ⅰ gene from oligo chip microarray analysis were increased over, but the expression of topoisomerase IIα and IIβ gene were decreased over. In contrast, the expression of topoisomerase Ⅱα and Ⅱβ gene were increased over in HL-60 cells treated with doxorubicin, whereas the expression of topoisomerase Ⅲα and Ⅲβ mRNA remained no significant change. These results suggest that these data may be useful for novel therapeutic markers.