Biosynthesis of elastin and other matrix-macromolecules in veinous arterial prosthesis.

: Homologous saphenous veins where implanted in the arterial circilation (femoral artery) of dogs for various times (1 to 9 months, most data given for 3 months). After this period, the in vitro biosynthesis of intercullar matrix macromolecules was studied by incubating the veins in organ culture conditions in the presence of 14C-lysine for 3 days. A fractional extraction procedure was used to obtain representative macromolecular extracts for the determination of chemical composition (hydroxyproline, hexosamines) and radioactivity. Neosynthesis of elastin was considered as valid criteria for the adaptation of the veinous wall to the new (arterial circulatory) conditions. The chemical composition of the grafted veins was different from that of the nongrafted, controlateral saphenous veins suggesting a molecular remodeling of the grafted veinous wall. Radioactive lysine was incorporated in all macromolecular fractions of grafted and control veins. The specific radioactivity of the extracts obtained from grafted veins was higher than that obtained from the control veins. The only exception was the collagenase extract (containing mainly the polymeric collagen) which had a somewhat lower radioactivity in the grafted vein. The incorporation of lysine in the elastin fraction increased by a factor 10 in the grafted vein as compared to the non-grafted veins. Radioactive desmosine could be demonstrated in the elastin fraction. It appears therefore that the veinous wall could adapt its biosynthetic capacity to the new circulatory conditions by increasing considerably the biosynthesis of elastin. This is accompanied by an increase of the synthesis of other macromolecules and a significant remodeling of the macromolecular structure of the vessel wall.