Diffusive analyte loss in capillary electrophoresis caused by delay in field application

In capillary electrophoresis with sample injection into the capillary mouth, delay between returning the capillary to the inlet reservoir and initiating solution mobilization allows analyte leakage by diffusion from the capillary mouth. This leakage has been modelled on the basis of either convective or diffusive analyte dispersal and the models have been compared with experimental results for peak diminution. Peak widths have also received attention and their utility in estimating analyte amounts has been investigated.