Molecular Mapping of Two Semidwarf Genes in an indica Rice Variety Aitaiyin3 (Oryza sativa L.)

The rice dwarf variety Aitaiyin3 is derived from a semidwarf cultivar Taiyin1.Genetic analysis indicated that the dwarf phenotype in Aitaiyin3 is involved in two recessive loci.Using bulk analysis with SSR markers,the two loci were located on the chromosome 1 and chromosome 4,respectively.Moreover,the allelism test showed that the locus on the chromosome 1 is allelic to the semidwarf gene sd1,while the other one is a new locus,here was named as sdt2.Further molecular mapping showed that sd1 was linked with SSR markers in the order of RM297-RM302-RM212-OSR3-sd1 with genetic distances of 4.7 cM,0 cM,0.8 cM and 0 cM,respectively.The linkage relationship of sdt2 with five SSR markers was SSR332-RM1305-sdt2-RM5633-RM307-RM401 with genetic distances of 11.6 cM,3.8 cM,0.4 cM,0 cM and 0.4 cM,respectively.