Binding and distribution of Na2B12H11SH on cellular and subcellular level in tumor tissue of glioma patients in boron neutron capture therapy

To determine binding and distribution of Na2B12H11SH (BSH)in glioma tissue in case of boron neutroncapture therapy, an antibody to this compound wasproduced and used in immunohistochemical investigations. It ispossible to trace BSH in immunohistochemistry, because BSHis firmly bound to the glioma tissue. Theantibody against BSH is specific for that antigen,as tumor tissue from patients without BSH administrationdid not stain. In areas of healthy brainfrom BSH infused patients, no staining of tissuewas detectable. In tumor tissues, BSH is presentingas a strong staining in cytoplasm and nucleusareas.