Molecular marker of gene for regulating amylose content of rice and application thereof

The invention discloses a molecular marker of a gene for regulating amylose content of rice and application thereof. The invention provides a method for identifying the rice with different amylose contents in an assisted manner, comprising the following steps: taking the genome DNA as a template and using WxM1 for PCR, determining the genetype of the rice as type I or type II if obtaining 252bp DNA and determining the genetype of the rice as type III if obtaining 275bp DNA; taking the genome DNA as a template, using WxM2 for PCR and Nhe I for enzyme digestion, determining the genetype of the rice as type I or type III if obtaining 1018bp DNA and determining the genetype of the rice as type II if obtaining 644bp and 374bp DNA; the amylose content of the rice with genetype being type III being lower than that of the rice with genetype being type I and the amylose content of the rice with genetype being type I being lower than that of the rice with genetype being type II; forming WxM1 bythe DNAs shown in the sequences 1 and 2; and forming WxM2 by the DNAs shown in the sequences 3 and 4. The invention has important theoretical value and practice significance in rice quality improvement, has direct application value in purposefully improving the rice quality by utilizing the genetic engineering technology or using the molecular marker to assist breeding and has theoretical value in studying biosynthesis mechanism of plant starch.