Expression of long pentraxin PTX3 in human adipose tissue and its relation with cardiovascular risk factors

Abstract Pentraxin 3 (PTX3) is an acute phase protein strongly expressed by advanced atherosclerotic lesions. We investigated (a) PTX3 expression and secretion in subcutaneous adipose tissue (SAT) and omental visceral adipose tissue (VAT) obtained from 21 obese (37.4±8.15yr) and 10 normal weight subjects (43.7±11.07yr) and (b) the relationships of adipose PTX3 with tumour necrosis factor α (TNFα) and adiponectin expression and with cardiometabolic risk factors. Real-time PCR was used to quantify specific mRNA for PTX3, CD68 (macrophage marker), TNFα and adiponectin. Fresh adipose tissue was cultured and PTX3 measured in the medium. Serum insulin, glucose, HDL and LDL cholesterol, triglycerides, C-reactive protein (CRP), fibrinogen, adiponectin, TNFα and PTX3 were measured. PTX3 expression was similar in the two fat compartments and tended to be higher in obese than in normal weight subjects in VAT only ( p =0.05). CD68 and PTX3 expressions were correlated with each other in SAT but not in VAT. After adjustment for age and sex, VAT-PTX3 expression and release were correlated with VAT-TNFα expression ( p p p p p Conclusions Human adipose tissue expresses and releases PTX3 likely under TNFα control. VAT production of PTX3 seems to contribute to the mechanisms underlying the development of atherosclerosis.