Utilization of vitamin B1 from fullers' earth adsorbates.
1937
We reported1 our results with various methods of eluting vitamin B1 from the international standard acid clay. The outcome of these experiments indicated that the vitamin on the acid clay was not entirely available to the test animal since the quinine sulfate extract was more effective than the clay itself in causing a remission of the symptoms of polyneuritis in rats. The experiments here reported were carried out to confirm those observations with a fuller's earth adsorbate of the vitamin.One hundred cubic centimeters of a solution of crystalline vitamin B1 containing 2500 curative doses, as determined by the Ammerman and Waterman2 modification of the Smith curative procedure, were treated with 20 gm. of English fuller's earth. The recovered fuller's earth after careful drying weighed 22 gm. The filtrate, together with a portion of this fuller's earth, was set aside for direct feeding to the test animals. Another portion of the earth adsorbate was extracted with quinine sulfate after the method of Willi...
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