[The expressions of periostin and the related factors during healing process of full-thickness cutaneous wound in rat].

Objective To observe the expression of osteoblast-specific factor 2 (periostin,PN),angiopoietin- 1 ( Ang- 1 ),vascular endothelial growth factor ( VEGF ) and vascular endothelial growth factor receptor-2 [ VEGFR-2/fetal liver kinase-1 ( FLK-1 )]in wound surface and its peripheral skin,and their effects on wound healing in rats.Methods Forty-eight Sprague-Dawley (SD) rats were randomly divided into six groups,with 8 rats in each group.An area of 2 cm × 2 cm full-thickness skin was excised on both sides of the back of rats.Specimens from wounds were obtained on 1,4,7,10,14,21 days after operation,and histological evaluation and immunohistochemical staining of PN,Ang-1,VEGF and FLK-1 were made to determine their expression levels.Normal skin specimens wereobtained as normal controls.Results The expressions of PN,Ang-1,VEGF and FLK-1 were significantly increased in wound surface after operation.Compared with the skin of normal controls,the expression of PN in the tissues of wound increased by 234.4％ on the 1 st day,and then increased continuously up to 597.9％ on the 7th day (reaching the peak )after operation,followed by a decrease,the increase rate was 280.9％ on the 21st day,and still remained at a high level (all P＜0.05 ).The expression of Ang-1 in the tissue of wound increased by 128.1％ on the 1st day and 327.5％ on the 4th day (reaching the peak),and then,it was gradually decreased.The increase rate was only 80.5％ on the 14th day and it rose slightly later ( all P＜0.05 ).The expression of VEGF in the tissues of wound reached the peak ( 165.8％ ) on the 7th day.Then it decreased with a slight fluctuation (all P＜0.05 ).The expression of FLK- 1 in the tissues of wound was increased by 56.1％ on the 1st day,and the level remained.It reached the peak by an increase of 70.1％ on the 7th day (both P＜0.05 ).Then,it was lowered after the 10th day ( all P＞0.05 ).Conclusions The expressions of PN,Ang-1,VEGF,FLK-1 were obviously increased during healing of skin wound,with different peaking time and expressing rates.The increase in expression of PN showed the longest duration and highest peak value.The PN,Ang-1,VEGF,FLK-1 all play a role in the wound healing process,and PN might play an important role during the healing process of a full-thickness cutaneous wound. Key words: Periostin; Wound healing; Angiopoietin-1; Vascular endothelial growth factor; Vascularendothelial growth factor receptor-2