Colorimetric Detection of Glucose in Biological Fluids Using Toner-Based Microzone Plates

This report describes the use of toner-based microzone plates for quantitative determination of glucose in artificial urine and human serum samples using colorimetric detection. The proposed approach has exhibited a linear response for glucose concentration levels from 0 to 10 mmol L-1 with determination coefficient (R2) of 0.996. The achieved values for sensitivity and limit of detection were 5.1 AU (mmol L-1)-1 and 0.6 mmol L-1, respectively. The glucose analysis in artificial serum samples revealed error lower than 5% in comparison with certified values provided by the supplier. Lastly, the proposed approach has also provided suitable accuracy (92-105%) for glucose measurements in artificial urine samples.