Electrophysiological properties of isolated rat liver cells.

The electrophysiological properties of isolated rat liver cells were studied using the patch clamp method in whole-cell configuration. The membrane potential in isolated hepatocytes was −42 ± 17 mV (n = 20). The input resistance (Rin) and the time constant (τm) were 51 ± 17 M (the range of 34 to 180 MΩ) (n = 20) and 4.2 ± 1.0 msec (the range of 3 to 16.5 ms) (n = 20). Assuming that the specific membrane capacitance is 1 μF/cm2, the membrane resistance and membrane capacitance were 42. ± 9.0 KΩ cm2 and 87 ± 27 pF. These values indicate that isolated rat hepatocytes are not abnormally permeable or leaky. The current-voltage relationship was linear with no rectification. The depolarizing pulse from the resting potential did not induce fast or slow inward currents even when norepinephrine or high Ca2 (3.6 mM) were applied. This indicates that there is no voltage-sensitive Ca2+ channel in the isolated hepatocytes.