Membrane connectivity as a robust measure for the HER2 IHC score

The use of digital image analysis for HER2 IHC evaluation is encouraged by ASCO/CAP and reimbursement policies. By individually analyzing the invasive tumor cells and determining the ratio of invasive tumor cells with positive membrane staining, the commercially available classical HER2 IHC algorithms for image analysis are compatible with the scoring principles of the ASCO/CAP guidelines.