An embedded single-cell impedametric positioning tracker in microfluidic deformability assays

The paper reports the integration of bioimpedance sensing and cell deformability assays in a unique microfluidic channel for label-free and image-free analysis of single-cell biophysical (biomechanical) attributes. The all-transparent microfluidic chip consists of a constriction channel and an array of embedded indium-tin-oxide (ITO) electrodes through which cell position can be tracked with high resolution. Therefore, information regarding cell transit times and velocities during cell deformation can be readily assessed without the use of video microscopy. The deformability chip is also unique in that it separates the delivery and sensing channel thereby preventing cell clogging. The performance of the chip has been verified by single-cell analysis of breast cancer cells.