Antibacterial Activities and Time Contact Green Tea Infussion (Camellia Sinensis) Againsts Salmonella Typhi by In Vitro

Green tea (Camellia sinensis) contains cathecin which has been reported to have various pharmacologic properties,such as an antibacterial agent. Salmonella typhi, as agent of typhoid fever, remains a public health problem in tropicalcountries; about 20 million cases and 600.000 deaths annually all over the world. Objectives of this research were toobserve the antibacterial activities and contact time of green tea infusion againsts Salmonella typhi by in vitroexperiment. The experiment took place in Microbiology Laboratory, School of Medicine, Padjadjaran University,Bandung, March-April 2009. Methods: In vitro laboratory analytic study has been conducted on green tea infusionof Indonesian and Japanese commercial package againsts Salmonella typhi. The study used agar well diffusionmethod and analyzed by ANAVA and t-independent test. Results: Only at concentration of 40% (w/v), Indonesiangreen tea infusion gave an average inhibition area of 3.376±0.334 mm diameter, and 3.571±0.217 mm on Japanesepackage, while below 40% were 0.707±0.000 mm with no differences between both packages (p>0.551). There hasbeen observed any turbidity in all Muller Hinton liquid media on both packages compared with control medium, alsoany growth of Salmonella typhi collony in all Muller Hinton agar at concentrations below 40%. Green tea infussionon both packages has been observed to have antibacterial activities at 40% but neither been observed at concentrationbelow 40%. [ MKB. 2010;42(2):51-5]. Key words: Green tea, infussion, antibacterial activities, time contact, Salmonella typhi