Simultaneous determination of metaclopramide and paracetamol by area under curve spectrophotometric method in combined tablet dosage form

The method for the simultaneous determination of Metaclopramide and Paracetamol by spectroscopy has been developed. The simple, accurate and precise method includes Area under the Curve (AUC) method. From a solvent effect studies and the spectral behaviors of Metaclopramide and Paracetamol, Milli-Q Water (DDW) was selected as solvent. Metaclopramide show maximum absorbance at 273 nm and Paracetamol shows maximum absorbance at 243 nm. The linearity range lies between 4-16mg/mL for Metaclopramide & Paracetamol at their respective wavelengths For the AUC method, the wavelength ranges between 268-278 nm and 238-248 nm were selected with reference to the absorbance curves plotted between the wavelengths of 200-400 nm. This method allows rapid analysis of two drug combination. The results of analysis were validated statistically and by recovery studies by following ICH method validation guideline. Tablet containing both drugs was assayed using the methods developed, showing a good accuracy and precision.